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Thursday, July 30, 2020 | History

2 edition of effect of steroids on mouse oocyte maturation in vitro found in the catalog.

effect of steroids on mouse oocyte maturation in vitro

C. Brent Barrett

effect of steroids on mouse oocyte maturation in vitro

by C. Brent Barrett

  • 139 Want to read
  • 5 Currently reading

Published .
Written in English

    Subjects:
  • Fertilization in vitro.,
  • Steroid hormones.,
  • Oocytin.

  • Edition Notes

    Statementby C. Brent Barrett.
    ContributionsBoston College. Dept. of Biology.
    The Physical Object
    Paginationxi, 122 leaves :
    Number of Pages122
    ID Numbers
    Open LibraryOL16576401M

    beneficial effect in increasing GSH concentrations in MII oocytes. Keywords: In Vitro Maturation, Crocin, Glutathione, Mouse, Oocyte Citation: Mokhber Maleki E, Eimani H, Bigdeli MR, Golkar Narenji A, Abedi R. Effects of crocin supplementation during in vitro maturation of mouse oocytes on glutathione synthesis and cytoplasmic maturation. with a total of 60 or more oocytes. Effect of puromycin on maturation in vitro Puromycin, at a concentration of 10 /tg/ml, is an effective inhibitor of meiotic maturation of mouse oocytes (Fig. 2) and dramatically reduces protein synthesis in these oocytes (Fig. 6). However, GV breakdown and chromosome condensation take.

    GVBD-oocytes, germinal vesicle brake down) are capable for successful in vitro maturation. After 24h to 48h of cultivation, about 80 to 90% of oocytes mature, i.e. riche the MfII stage of nuclear.   In vitro maturation (IVM) is a technique used to induce immature oocytes collected in different periods of embryonic growth. The rates vary for immature oocytes collected from different clinical sources to potentially develop into embryos and achieve live birth. As an effective treatment method, IVM can be used to treat patients with polycystic ovary syndrome (PCOS), ovarian.

    Marrs RP, Saito H, Yee B, Sato F, Brown J () Effect of variation of in vitro culture techniques upon oocyte fertilization and embryo development in human in vitro fertilization procedures. Fertility and Sterility – initiate nuclear maturation in vitro. Similarly, grow- ing Xenopus or starfish oocytes do not respond to pro- gesterone or to l-methyl adenine (Wasserman et al., ; Bulet et al., ) by resuming meiosis. Since the lack of meiotic response to progesterone by late stage IV Xenopus oocytes is not due to the absence of steroid.


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Effect of steroids on mouse oocyte maturation in vitro by C. Brent Barrett Download PDF EPUB FB2

In the present study, we examined the effect of progesterone on survival, in vitro growth and in vitro maturation of follicles and the maturation of oocytes derived from mouse polycystic ovary. Beloosesky et al. indicated following 14 days of testosterone injection, progesterone concentration in the testosterone treated was only 20% of control Author: Bazdar A, Azadbakht M.

During mouse COCs maturation in vitro, FSH promotes oocyte nuclear maturation and cumulus-cell expansion mainly by activating aromatase, transforming androstenedione into E 2, and triggering GPRdependent phosphorylation of ERK1/2; this ultimately promotes the expansion of cumulus cells and the maturation of : Hui Zhao, Junbang Ge, Junbang Ge, Juncai Wei, Jie Liu, Chen Liu, Chiyuan Ma, Xiaoe Zhao, Qiang Wei.

Effects of steroids on mouse oocyte maturation in vitro. Smith DM, Tenney DY. Oestrone and dihydrotestosterone had no significant action. Other steroids inhibited maturation. The stage of maturation most affected and the median effective concentration (MEC) at this stage varied with different by: Effect of steroids on oocyte maturation and atresia in mouse ovarian fragments Article (PDF Available) in J Reprod Fertil 58(1) February with 19 Reads How we measure 'reads'.

The stage of maturation most affected and the median effective concentration (MEC) at this stage varied with different steroids. The predominant effect of pregnenolone (MEC = microM), progesterone (MEC = microM), androstenedione (MEC = microM) and oestradiol beta (MEC = microM) was to block maturation after the resumption Cited by: CiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): Summary.

Oestrone and dihydrotestosterone had no significant action. Other steroids inhibited maturation. The stage of maturation most affected and the median effective concentration (MEC) at this stage varied with different steroids.

The predominant effect of pregnenolone (MEC = 6\m.\4 \g=m\m),progesterone (MEC = 5. Effects of steroids on mouse oocyte maturation in vitro in Reproduction. Authors: The stage of maturation most affected and the median effective concentration (MEC) at this stage varied with different steroids.

The predominant effect of pregnenolone. Throughout the life of female mammals, only a small number of viable oocytes are produced. The mechanisms underlying the creation and selection of competent oocytes remain unclear.

Here, we propose a novel approach for elucidating these unsolved questions, involving the use of an in vitro system established in the present study, which can fully reproduce mammalian oogenesis from mouse. Although cortisol and dexamethasone had little effect on oocytes in the mouse, corticosterone (CORT) The effect of glucocorticoids on mouse oocyte in vitro maturation and subsequent fertilization and embryo development.

The brain is an important target of adrenal steroid actions. A comparison of synthetic and natural steroids. We first investigated any abrupt effect of oocyte contact to DES in vitro.

Mouse oocytes were cultured with different concentrations (0, 20, 40, 60 μM) of DES, and the PBE rate was examined after 14 h culture.

As shown in Fig. 1A, DES exposure adversely affected oocytes maturation. In mouse cumulus cells, a heat-stable meiosis activating substance is stimulated by FSH (Byskov et al., ; Yding Andersen et al., ) and artificial elevation of MAS in cumulus cells using inhibitors of specific enzymes on the steroid synthesis pathway promotes maturation of mouse oocytes in vitro (Leonardsen et al., ).

History. InPincus & Enzmann did the first experiment on immature rabbit oocyte, showing in vitro spontaneous maturation and fertilization. They showed maturation occurs in isolation from normal follicular environment. In Edwards then continued IVM studies in mouse, sheep, cow, pig, rhesus monkey and human.

Andersen C () Effect of glucocorticoids on spontaneous and follicle- stimulating hormone induced oocyte maturation in mouse oocytes during culture. J Steroid Biochem Mol Biol 85(): Macedo G, Zuccari C, De Abreu U, Negrao J, Da Costa e Silva E () Human-animal interaction, stress, and embryo production in Bos indicus embryo.

Antioxidants improved oocyte maturation after pre-maturation aging. To investigate the effects of antioxidants on the competence of oocytes that matured to metaphase II (MII) stage, oocytes. The in vitro grown oocytes were cultured for a further 22–24 h for oocyte maturation; 13% and 30% of oocytes grown in the 10 and ng/ml 17β-estradiol-supplemented medium reached metaphase II, respectively; more than 64% of oocytes grown in the androstenedione-supplemented medium matured to metaphase II.

These results show that. In vitro maturation of oocytes. In vitro grown oocytes were denuded completely by pipetting with a narrow-bore pipette. The denuded oocytes were each transferred into a microdrop (10 μl) of the culture medium to be measured for size, and cultured for 24 hr to allow spontaneous maturation.

The effects of hyper- and hypo-glycaemic conditions during the in vitro maturation of mouse cumulus–oocyte complexes on developmental competence were examined, with an emphasis on the role of the hexosamine biosynthesis pathway.

A low (1 mM) glucose concentration achieved optimal oocyte competence (3-fold higher blastocyst development rate compared with high (30 mM) glucose.

Whereas some studies observed that glucose metabolic activity during oocyte maturation was correlated well with embryo development after insemination in vi17,19,20,21, the effect of glucose. The purpose of this study was to determine if the addition of steroid hormones into the culture medium could influence the in vitro maturation of pig oocytes.

The cumulus-oocyte complexes (COCs). collected from follicles of mm diameter, were matured in steroid-free medium supplemented with various concentrations of estradiolbeta ( Progesterone is widely used to induce maturation of isolated fully grown oocytes of the African clawed frog, Xenopus laevis.

However, the hormone fails to release oocytes from the layer of surrounding follicle cells. Here, we report that maturation and follicle rupture can be recapitulated in vitro by treating isolated follicular oocytes with progesterone and low doses of the matrix.

There is increasing interest in the use of in-vitro-matured (IVM) oocytes as a clinical treatment. IVM oocyte treatment. for infertile women has several advantages, including the. elimination of side effects associated with gonadotrophin. Article Effect of gonadotrophin stimulation on mouse.

oocyte quality and subsequent embryonic.Abstract. Purpose: Maturation promoting factor (MPF) acts at theresumption of meiosis and nonspecifically throughout theanimal species.

There exists a considerable body of literatureon MPF, but little work has been done to study the inductionof maturation of mammalian oocytes by microinjection ofextracted MPF.The effects of a prolonged culture with PDE3-I on oocyte quality prior to and after reversal from the inhibition are not known.

This study tested the impact of long-term in vitro exposure of two PDE3-Is, org and cilostamide, on oocytes using a mouse follicle culture model.